Detection of GCA Transforming Growth Factor β Receptor I, II Methylation Status and its Clinical Significance 

Hong Wei, Guangmei Kang, Junxia Gu, Jianxia Liu, Hongyan She, Jing Zhang, Wei Guo, Hongqi Gao, Yongsha Li, Wenjing Zhao 

Affiliated Hospital of Hebei University of Engineering, Handan, 056002, China 

Abstract: To investigate the levels of cardia adenocarcinoma (GCA) transforming growth factor β receptor І, II (TβR I, II) gene promoter methylation and mRNA expression in order to study its relationship with GCA and its possible mechanism. The advanced methylation specific polymerase chain reaction (MSP) and reverse transcription - polymerase chain reaction (RT-PCR) technique was used to detect TβR I, DNA methylation gene II and mRNA expression levels in cancer tissues and adjacent normal tissues. In 160 cases of GCA tis-sues, TβR I gene methylation rate was 52.3%, and the corresponding adjacent normal tissue methylation was 40.7%. The rate of methylation between the two groups showed no statistical significance (P> 0.05); In 160 cases of GCA tissues, TβR II gene methylation rate was 70.8%, and the corresponding adjacent normal tissue methylation was 47.5%. The rate of methylation between the two groups showed statistical significance (P <0.05); TβR I gene mRNA relative expression levels in GCA and adjacent normal tissues were 0.77 ± 0.17 and 0.99 ± 0.31. Its expression level in GCA group was significantly lower than that in adjacent group with statistical significance (P <0.05); TβR II gene mRNA relative expression levels were 0.89 ± 0.30 and 1.10 ± 0.29 in GCA and cancer adjacent normal tissues. Its expression level in GCA group was significantly lower than that in adjacent group with statistical significance (P <0.05); In GCA tissue mRNA expression level of the two genes showed no linear correlation (P> 0.05). TβR II gene methylation may correlate with occurrence of GCA. TβR I, II mRNA aberrant expression may correlate with the occurrence of GCA. 

Keywords: GCA; Methylation; MRNA; TβR I; TβR II