Cloning and Expression of Deep Red Fluorescent Protein Gene

Shuyan Song

Tianjin Agricultural University, Tianjin, 300384, China

Abstract: The deep red fluorescent protein gene DsRed2 was extracted from the original plasmid to form a prokaryotic expression vector, and the clone and expression test of the deep red fluorescent protein gene was carried out. The test results showed that the fluorescent protein gene could be cloned and cultured in liquid culture medium at 37℃ for 1 to 5 hours, and it had strong chromogenic ability. Deep red fluorescent protein can easily distinguish bacterial colonies between positive and false positive and help cell localization.

Keywords: Deep red fluorescent protein; Protein gene cloning; Protein gene expression