Acute Toxic Effects of Chlorpyrifostrichlorphon Mixture and Acetochlor on Bufo Gargarizans Tadpoles

1*Mingwang Zhang, 1Xinghai Jia, 2Zhimin Wei, 1*Mingxian Yang 1Huailiang Xu, 1Yongfang Yao, 1Qingyong Ni, 1Linjie Wang

1College of Animal Science and Technology, Sichuan Agricultural University, 625014 Ya’an, Sichuan, P.R. China

2Institude of Millet Crops, Hebei Academy of Agriculture and Forestry Sciences, Cereal Crops Research Laboratory of Hebei Province, Shijiazhuang 050035, Hebei, R.R.China

Abstract: The acute toxic effects of two pesticides on Bufo gargarizans tadpoles were investigated in order to provide a scientific basis for the monitoring of aquatic environments and proper use of pesticides. Experimental methods designed to determine the acute effects of specific agents on aquatic organisms were used to explore acute toxic effects of single exposure of chlorpyrifos-trichlorphon mixture and acetochlor on Bufo gargarizans tadpoles. For chlorpyrifos-trichlorphon mixture, at 24, 48, 72, and 96 h, median lethal concentrations (LC50) for Bufo gargarizans tadpoles were 12.023, 7.499, 4.977, and 2.799 ml/L, while for acetochlor were 0.064, 0.049, 0.043, and 0.026 mg/L. The safe concentrations (SC) were 0.875 ml/L and 0.009 ml/L for chlorpyrifos- trichlorphon mixture and acetochlor, respectively. These results show that the toxicity of acetochlor against B. gargarizans tadpoles was more toxic than that of chlorpyrifos- trichlorphon mixture, and it will provide a basis for proper use of these two pesticides.

Keywords: Chlorpyrifos-trichlorphon Mixture; Acetochlor; Bufo Gargarizans Tadpoles; Acute Toxicity